Bone Tissue Engineering Utilizing Adult Stem Cells in Biologically Functionalized Hydrogels

The goal of these studies was to investigate the effect of resveratrol treatment on the osteogenic potential of human and rat adipose derived stem cells both in a 2-D and 3D culture environment. Adipose derived stem cells (ADSCs) have shown promise as a potential source of osteogenic progenitor cells. Resveratrol has been shown to inhibit adipogenic differentiation while simultaneously activating osteogenic differentiation. 2D experiments with rat ADSCs identified 25 μM of resveratrol as an effective dose to increase the production of osteogenic genes. The effect of 25 μM resveratrol treatment was investigated in a pre-treatment and continuous treatment regimen in 3-D culture and demonstrated that pre-treatment was sufficient to increase the mineralized matrix production of rat ADSCs. We next sought to investigate whether this result was also observable with human ADSCs and found that the human cells did not respond to 25 μM